Types of PEGylation of Peptides
Release date：2020/1/17 13:06:35
Polyethylene glycol (PEG), also known as polyethylene oxide (PEO), is an amphiphilic polyether that is soluble in water and most organic solvents. PEG and its derivatives are one of the few polymers that can be used in biopharmaceutical products certified by the US FDA.
PEGylation refers to the covalent attachment of a PEG polymer chain to a target molecule, usually a small molecule chemical drug or a large biomolecule such as a peptide, protein, carbohydrate, lipid, oligonucleotide, affinity ligand, cofactor, Liposomes and other biological materials.
The application of the peptide molecule as a drug lead compound in the body is limited to a certain extent, mainly in the filtering action of the glomerulus of the organism, the hydrolytic destruction of the protease, and the antigen response. After the polypeptide is modified with PEG (PEGylation), the restrictions in the above three aspects are greatly reduced, thereby improving the application of the polypeptide in vivo.
The main modification sites of the PEGylated polypeptide are the N-terminus, C-terminus of the polypeptide, the side chain of Lys and the thiol group of Cys. The molecular weight range of the monodispersed PEG used for the modification is between PEG2 ~ PEG24; the molecular weight range of the polydispersed PEG is between PEG500 ~ PEG40K.
Related: PEGylation Reagents Selection Guide
1. Introduction to common monodispersed PEG modifications (all the following structures can be modified to the polypeptide structure)
2. Introduction to common polydispersed PEG modifications (all the following structures can be modified to the polypeptide structure)