Lipid-Specific QD Labeling of Enveloped Viruses Using DSPE-PEG-Biotin
Release date：2020/3/26 7:11:47
Quantum dots (QD) have optical properties of super bright fluorescence, excellent photostability, narrow emission spectra and optional colors. QD-labeled single molecules / viruses can be continuously and quickly imaged for a long time, and more detailed information provided than other fluorophore-labeled. QDs have rarely been used to study viral infections, mainly due to the lack of a recognized labeling strategy. This study developed a lipid-specific method to readily, mildly, specifically, and efficiently label enveloped viruses with quantum dots by recognizing viral envelope lipids with lipid-biotin conjugates (DSPE-PEG-Biotin) and recognizing these lipid-biotin conjugates with streptavidin-quantum dot conjugates.
Such a strategy can thoroughly evade ultracentrifugation, dialysis, and ultrafiltration processes that are indispensable for removing the cell-derived reactive molecules, redundant functional reagents, unlabeled viruses, or unbound QDs in many other labeling strategies. This strategy furthest minimized and simplified the handling of viruses, making the QD labeling milder and more convenient.
Biochempeg now offers an extensive group of PEG-lipid conjugates (DSPE PEG) incorporating various functionalized PEG terminal, like Biotin, Amine, Carboxylic acid, Azide, Aldehyde, Thiol, and Hydroxy.