Professional PEGylated Protein Development Services
From recombinant protein expression to high-purity PEG conjugation, Biopharma PEG provides validated, scalable, end-to-end solutions for biopharmaceutical development.
Service Overview
PEGylation is an advanced biopharmaceutical technology that covalently attaches PEG chains to protein surfaces, significantly improving pharmacokinetics, stability, and immunogenicity. Biopharma PEG provides integrated PEGylation solutions covering recombinant protein expression, PEG-protein conjugation, purification, and analytical characterization.
Our platform supports multiple PEG conjugation chemistries with consistent quality, making it suitable for preclinical and clinical-stage drug development.
Core capabilities:
- ● Recombinant Protein Expression (E.coli/yeast, ≥95% purity)
- ● Multiple PEGylation Chemistries (Aldehyde, NHS, Maleimide, site-specific)
- ● Comprehensive QC (SEC-HPLC, RP-HPLC, SDS-PAGE, MALDI-TOF, endotoxin, peptide mapping)
Recombinant Protein Production
We have established mature prokaryotic (E. coli) and eukaryotic (yeast) expression systems and have successfully expressed more than 100 proteins for PEGylation projects and drug-research applications.
Representative proteins:
- ● G-CSF: Purity ≥95%, Specific activity: 9.28×10⁷ IU/mg, Endotoxin <30 EU/mg
- ● Human Growth Hormone (hGH): Purity ≥95%, Specific activity: 18 IU/mg
- ● Interferon α-2a: Purity ≥95%, Specific activity: 2.8×10⁸ IU/mg
PEGylation Technology Platform
We provide PEGylation at N-terminus, C-terminus, lysine, cysteine, and other amino acid residues.
Custom conjugation strategies can be designed based on protein structure and function.
Available PEGylation Chemistry:
- ● NHS-ester conjugation (lysine side chains)
- ● Aldehyde conjugation (N-terminus)
- ● Maleimide conjugation (cysteine residues)
- ● Custom site-specific PEGylation (tailored to project needs)
Representative PEGylated Protein Projects
Case 1 – PEGylated G-CSF (mPEG20K-Aldehyde)
- ● Biological Activity: 1.56 × 10⁸ IU/mg
- ● Purity (SDS-PAGE): 96%
- ● Purity (HPLC): 97.1%
- ● Purity (SEC-HPLC): 100.0%
- ● Molecular Weight: 38.9 kDa
- ● Bacterial Endotoxin: ≤0.25 EU
- ● Peptide Mapping: Consistent
- ● Free PEG: Not detected
Case 2 – PEGylated hGH (mPEG-NHS)
- ● Biological Activity: 8 IU/mg
- ● Purity (SDS-PAGE): 96.8%
- ● Purity (HPLC): 97.4%
- ● Purity (SEC-HPLC): 100.0%
- ● Molecular Weight: 60.8 kDa
- ● Peptide Mapping: Consistent
- ● Free PEG: Not detected
Case 3 – PEGylated IFN-α2a (Y-mPEG40K / U-mPEG40K-NHS)
- ● Protein Content (SEC-HPLC): 94.7%
- ● Specific Activity: 3.4 × 10⁸ IU/mg
- ● Purity (SDS-PAGE): 98.5%
- ● Purity (HPLC): 97.5%
- ● Molecular Weight: 62.8 kDa
- ● Peptide Mapping: Consistent
- ● Free PEG: 0.4%
PEGylation Workflow
- ● Project Evaluation
- ● Protein Expression or Customer-Provided Protein
- ● PEG Selection & Conjugation Strategy Design
- ● PEGylation Reaction
- ● Purification (SEC-HPLC / Ion exchange chromatography (IEC) / Hydrophobic Interaction Chromatography (HIC) )
- ● Quality Testing and Final Project Delivery
PEG Linkers Available
- ● Linear PEG (monodispersed & polydispersed)
- ● Y-shaped PEG
- ● Multi-arm PEG ( 4-arm / 8-arm)
- ● High-purity GMP-grade PEGs
We can also customize PEG molecular weight, reactive groups, and linker structures based on project needs.
Start Your PEGylation Project Today!
Our team is ready to support your protein modification needs with high-quality PEG solutions.
Email: sales@biochempeg.com