Click Chemistry

Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching fluorophores and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. 

One of the earliest and most important methods in bioconjugation was to express a reporter on the same open reading frame as a biomolecule of interest. Notably, GFP was first (and still is) expressed in this way at the N- or C- terminus of many proteins. However, this approach comes with several difficulties. For instance, GFP is a very large unit and can often affect the folding of the protein of interest. Moreover, by being expressed at either terminus, the GFP adduct can also affect the targeting and expression of the desired protein. Finally, using this method, GFP can only be attached to proteins, and not post-translationally, leaving other important biomolecular classes (nucleic acids, lipids, carbohydrates, etc.) out of reach.

Now limitations emerge from the chemistry of the probe to its target. In order for this technique to be useful in biological systems, click chemistry must run at or near biological conditions, produce little and (ideally) non-toxic byproducts, have (preferably) single and stable products at the same conditions, and proceed quickly to high yield in one pot. Existing reactions, such as Staudinger ligation and the Huisgen 1,3–dipolar cycloaddition, have been modified and optimized for such reaction conditions. Today, research in the field concerns not only understanding and developing new reactions and repurposing and re-understanding known reactions, but also expanding methods used to incorporate reaction partners into living systems, engineering novel reaction partners, and developing applications for bioconjugation.

Biochempeg is especially good at click chemistry reagents which now widely used on biomolecular probes, two-dimensional gel electrophoresis separation, modification of peptide function with triazoles and natural products, and pharmaceuticals, nanotechnology, Biomaterials, etc. Biochempeg provides high purity Click Chemistry tools with a broad range of functional groups: Azide, Alkyne, DBCO, BCN... (DBCO PEGs for Copper-Free Click Chemistry)

Technique support: GMP standard production support, parameter control. Contact for Technical Files.

Catalog No. Name Structure M.W. Purity
MD006016 N3-PEG2-COOH N3-PEG2-COOH 203.19 ≥95%
MD006016 N3-PEG12-COOH N3-PEG12-COOH 643.72 ≥95%
MD007002 Alkyne-PEG5-OH Alkyne-PEG5-OH 276.3 ≥95%
MD007006 Alkyne-PEG9-N3 Alkyne-PEG9-N3 ≥95%
MD007056 Alkyne-PEG8-Br Alkyne-PEG8-Br ≥95%
MD007067 Alkyne-PEG5-CH2CH2COOtBu Alkyne-PEG5-CH2CH2COOtBu 360.4 ≥95%
MD048005 DBCO-PEG4-NH2 DBCO-PEG4-NH2 523.626 ≥95%