Release date:2020/5/14 11:01:56
Click Chemistry has found a wide range of applications in bioconjugation in the pharmaceutical and biotechnology industries. It is a term introduced by KB Sharpless in 2001 to describe a high yield, a wide range reaction that only produces by-products. These by-products can be separated without chromatography, are stereospecific, easy to perform, and can be conducted in easily removable or benign solvents. 

Biochempeg provides many
PEG Azide and PEG Alkyne derivatives for the conventional click chemistry reaction with copper (I) (CuAAC), but due to the cytotoxicity of the catalyst, the application of this reaction in biomedical systems is limited.

The novel copper-free click chemistry is based on the reaction of a cyclooctyne (DBCO) moiety with an azide-labeled reaction partner (called strain-promoted alkyne azide cycloaddition (SPAAC)). This new "click chemistry" is very fast at room temperature and does not require cytotoxic Cu (I) catalysts. Cyclooctyne has high thermal stability, very narrow and specific reactivity with azide, resulting in almost quantitative yields of stable triazoles. This unique covalent bond is generated when DBCO incorporated into one biomolecule reacts with the azide linker incorporated into the second biomolecule.

Features and Advantages Of Copper-Free Click Chemistry

• Biocompatibility – no cytotoxic copper catalyst is required – good in vivo application.
• Mild conditions –conjugation in an aqueous buffer medium and at low temperatures
• Stability – DBCO and azide moieties are stable for a long time
• Efficiency – stable triazole formation in quantitative yield
• Specificity and biological orthogonality – Azides only react with DBCO in the presence of -NH2, -SH, -COOH and other protein functionalities
However, most cyclooctyne reagents do not have good water solubility, which limits their application in biomedicine. Compared with cyclooctyne, Biochempeg's
DBCO PEG derivatives have good water solubility, and have better biocompatibility and no cytotoxicity than traditional click reagents. Biochempeg has enhanced its ability to provide high-quality PEG DBCO products in large kilograms and above at very affordable prices.

The following are a few examples of PEG DBCO products produced by Biochempeg. PEG and monodisperse PEG DBCO derivatives with different branches, molecular weight and functionality can be obtained by
custom synthesis.


Methoxy PEG DBCO Products (mPEG-DBCO)

Name M.W. Unit Price (USD)
mPEG-DBCO-1K 1K 100mg $195.00
mPEG-DBCO-1K 1K 1g $995.00
mPEG-DBCO-2K 2K 100mg $195.00
mPEG-DBCO-2K 2K 1g $995.00
mPEG-DBCO-5K 5K 100mg $195.00
mPEG-DBCO-5K 5K 1g $995.00
mPEG-DBCO-10K 10K 100mg $195.00
mPEG-DBCO-10K 10K 1g $995.00
mPEG-DBCO-20K 20K 100mg $195.00
mPEG-DBCO-20K 20K 1g $995.00

Homobifunctional PEG DBCO Products (DBCO-PEG-DBCO)

Name M.W. Stock Price (USD)
DBCO-PEG-DBCO-2K 2K In Stock Inquiry

Multi-arm PEG DBCO Products (4-ArmPEG-DBCO, 8-ArmPEG-DBCO)

Name M.W. Stock Price (USD)
4-ArmPEG-DBCO-10K 10K In Stock Inquiry
4-ArmPEG-DBCO-20K 20K In Stock Inquiry
8-ArmPEG-DBCO-20K 20K In Stock Inquiry

Heterobifunctional PEG DBCO Products (PEG-DBCO)

Name M.W. Price (USD)
NH2-PEG-DBCO 800, 1K, 2K, 3.4K, 5K, 10K Inquiry
DBCO-PEG-OH 500, 2K Inquiry
DBCO-PEG-SH 400, 600, 1K, 2K, 3.4K, 5K Inquiry
DBCO-PEG-COOH 1K, 2K, 3.4K, 5K Inquiry
DBCO-PEG-Silane 1K, 2K, 3.4K Inquiry
DBCO-PEG-Mal 1K, 2K, 3.4K, 5K, 10K, 20K Inquiry
DBCO-PEG-NHS 200, 400, 500, 600, 800, 1K, 2K, 3.4K, 5K, 6K, 8K, 10K, 20K Inquiry
DBCO-PEG-Biotin 200, 400, 500, 600, 800, 1K, 2K, 3.4K, 5K, 6K, 8K, 10K, 20K Inquiry
DBCO-PEG-FA 1K, 2K, 5K Inquiry
And More...    
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