• Synonyms：
  SC-PEG-SC
  Succinimidyl ester-PEG-Succinimidyl ester
  
• Recommended Storage Condition：
  
• Uses：
  

NHS-PEG-NHS is an amino (-NH2) reactive PEG derivative, which can be used to modify proteins, peptides or any other surface.

The NHS ester reacts with the primary amine group of pH 7~8.5 to form a stable amide bond. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to alkaline pH to proceed. Primary amines react with NHS esters through nucleophilic attack, and NHS is released as a by-product. The competition between the hydrolysis of NHS-ester and the reaction in the aqueous solution increases with the increase of pH.

Compared with other NHS-activated PEGs, the succinimide carbonate (SC) functionalized mPEG-NHS of NHS-PEG-NHS provides excellent reactivity and higher stability in aqueous solutions.

Biopharma PEG offers a wide range of NHS-activated PEGs from lab to commercial scale in both non-GMP and GMP grades. Email at [email protected] and start using a superior product for your next product R&D project.

Cited Publications

Our  NHS-PEG-NHS derivative has been cited in peer-reviewed scientific publications. Browse the references below to learn more.

1. Verdoliva, Valentina; Digilio, Giuseppe; Iaccarino, Emanuela; Luca, Stefania De (2025). Solvent-Free procedure of an A9 Peptide Dimer Exhibiting Specific HER2 Receptor Binding: Fluorescence Spectroscopy Evaluation of the Enhanced Binding Affinity. ACS Publications. Journal contribution. https://doi.org/10.1021/acs.jmedchem.5c01194.s001
2. Faour, Sara and Vézy, Cyrille and Déturche, Régis and Dedieu, Stéphane and Sohier, Jérôme and Jaffiol, Rodolphe Yvan, Surface Tension-Driven Persistence: How Hydrogel Interfacial Properties Regulate Fibroblast Directional Migration. Available at SSRN: https://ssrn.com/abstract=5354082 or http://dx.doi.org/10.2139/ssrn.5354082​

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